Custom Protein Service in Bacteria

EuzLab has organized a team of biologists who specialize in protein expression and purification in all four commonly used expression systems including bacteria, yeast, insect/baculovirus and mammalian cells. We proudly offer high quality recombinant protein services with the best price guarantee and quick turnaround time. It is our goal to accelerate innovative research by being a reliable partner in your discovery.

Escherichia coli (E. coli) is one of the most widely used hosts for the production of heterologous proteins. Its genetics are far better characterized than those of any other microorganism. E.coli expression is by far the most cost-efficient and consistent production method. We have the capabilities to scale up from milligrams to grams. Although E.coli do not incorporate post-translational modifications into the recombinant protein, many E.coli produced proteins do show activity of the native protein (our robust catalogue of active recombinant proteins is evidence of this). However bacterial cells produce endotoxins, so if you plan to use the protein in any animal cells or assays, endotoxin removal is recommended.

Table 1: Guaranteed Packages

Description
Deliverables
Turnaround
Price
‐ 0.5 to 1L Expression and purification
‐ Guaranteed 1 mg of purified protein
‐ QC Testing (SDS-PAGE and Western Blot)
‐ Weekly progress reports
‐ 1 mg of purified protein
‐ QC data
2‐3 weeks


$1,450


‐ 1L Expression and purification
‐ Guaranteed 3 mg of purified protein
‐ QC Testing (SDS-PAGE and Western Blot)
‐ Weekly progress reports
‐ 3 mg of purified protein
‐ QC data
2‐3 weeks


$1,899


‐ 1 to 2L Expression and purification
‐ Guaranteed 5 mg of purified protein
‐ QC Testing (SDS-PAGE and Western Blot)

‐ Weekly progress reports
‐ 5 mg of purified protein
‐ QC data
2‐3 weeks


$2,110


‐ 2 to 3L Expression and purification
‐ Guaranteed 15 mg of purified protein
‐ QC Testing (SDS-PAGE and Western Blot)
‐ Weekly progress reports
‐ 15 mg of purified protein
‐ QC data
4‐6 weeks


$2,880


NOTE:
1. Pricing applies only if gene construct is provided. Gene synthesis services are available for an additional cost.
2. The size of the target protein must be less than 100 kDA.
3. In rare cases, we may use alternative tags to purify a protein. The final protein may be tag containing.
4. Pricing does not include tag removal or endotoxin removal.Additional charges apply for these services.
5. We guarantee protein solubility but DO NOT guarantee the bioactivity or functionality of the protein. If the protein expresses as inclusion bodies, refolding will be done to make the protein soluble.
6. Quantity guaranteed or it is FREE.
7. Western Blot data is available FREE of charge for any tagged proteins than can be detected by the anti-HIS antibody. Additional charges apply for all other proteins.

Table 2: Custom Package

Description
Deliverables
Turnaround
Price
‐ Provide your expression constructs, or we’ll sub-clone your gene into our expression vector.
‐ Weekly progress reports
Please inquireFrom $1600
‐ Expression pilot study: expression optimization and verification.
‐ Purification Options:
A. We follow your purification protocol.
B. We choose the best course to purify your protein.
‐ Large scale expression and purification as desired (mg to g).
‐ Tag removal, refolding, endotoxin removal, etc. according to your requirements.
‐ QC testing: SDS‐PAGE and protein concentration (Western Blot)
‐ Tag removed,   purified protein
‐ QC data
Please inquire
Please inquire
NOTE: Western Blot data available FREE of charge for any tagged proteins than can be detected by the anti-HIS antibody. Additional charges apply for all other proteins.

Table 3: Intact Native Packages

Description
Deliverables
Turnaround
Price
- Sub-cloning into expression vector
- Expression pilot study: expression optimization and verification
- 1 mg purified protein, native form
- Large scale expression (2 to 3L) and purification
- Tag removal
- QC testing: SDS-PAGE and protein concentration (Western Blot)
- Weekly progress reports
- 1 mg of tag removed, purified,
- Protein in its native form
- 90 to 95% purity
- Endotoxin level <1eu/ug
- QC Data
4-6 weeks





$2,499





- Sub-cloning into expression vector
- Expression pilot study: expression optimization and verification
- 5 mg purified protein, native form
- Large scale expression (5 to 10L) and purification 
- Tag removal
- QC testing: SDS‐PAGE and protein concentration (Western Blot)
- Weekly progress reports
- 5 mg of tag removed, purified,
- Protein in its native form
- 90 to 95% purity
- Endotoxin level <1eu/ug
- QC Data
4-8 weeks





$5,200





NOTE: Western Blot data available FREE of charge for any tagged proteins than can be detected by the anti-HIS antibody. Additional charges apply for all other proteins.

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