Prestained Protein Ladder


Prestained Protein Ladder

Cat No.:P3001-250ul

Storage temperature at -20oC or at 4oC for 2 months.


Storage Buffer

67mM Tris-HCl, pH7.5, 20 mM DTT, 5mM EDTA, 2% (W/V) SDS, 33% (W/V) Glycerol, 0.02% (W/V) proclin 300.



Prostained Protein Ladder is a 2-color protein molecular weight standard containing 8 prestained recombinant proteins covering a wide range molecular weights from 15 kDa to 150 kDa. The ladder contains one orange reference band of ~70 kDa. Other bands are blue.

The ladder is supplied in 1xSDS-PAGE loading buffer and is ready-to-use without heating.



  1. Monitoring of protein separation during SDS-PAGE.
  2. Verifying Western transfer efficiency.
  3. Approximate sizing of proteins on SDS-polyacrylamide gels and Western blots.
  4. Locating a protein of interest for excision from an unstained preparative gel.



Recommendations for Loading

  1. Thaw the ladder at room temperature for a few minutes to dissolve precipitated solids. Do not boil!
  2. Mix gently, but thoroughly, to ensure the solution is homogeneous.
  3. Load the 5μl of the ladder on an SDS-polyacrylamide gel, and use the same volume for Western blotting.
  4. Each lot of the Prestained Protein Ladder is calculated apparent molecular weights as in the picture below.
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